GCSE Biology: Culturing Micro-Organisms

5th April 2017

GCSE Biology Revision Tips

Culturing Microorganisms


Bacteria divide through simple cell division. This process can happen as regularly as every 20 minutes if they have enough nutrients and a suitable temperature. Bacteria can grow in a nutrient broth or as colonies on an agar gel plate.

The purpose of culturing uncontaminated cultures is to investigate the function of antibiotics and disinfectants.

To prepare an uncontaminated culture using an aseptic technique you would use the follow steps:

  • Sterilise all culture media and petri dishes

  • Sterilise inoculating loops used to transfer microorganisms to the media

  • Carefully seal petri dish lid

  • Incubate at 25 degrees


We carry out this process to help us work out which bacterias cause certain diseases. In 1878, Robert Koch discovered which bacterias caused TB and cholera.


This is an important topic to understand if you want to pass your GCSE Biology. Revise this information and make sure it’s stuck in your head for the exam by listening to our GCSE Culturing Microorganisms song which you can find here.


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